C3 Protein

Purified human C3 protein


Product Specifications

Citations 17
Description

Purified human C3 protein

Storage Store at -70°C or below. Avoid freeze/thaw (>10 % activity loss per thaw).
Form

Frozen liquid. 10 mM sodium phosphate, 145 mM NaCl, pH 7.2. No preservative, 0.22 µm filtered.

Concentration 1.0 mg/mL (refer to CoA for lot specific concentration)
Applications

See citations and technical data sheet for application info.

Ordering Information

For Research Use Only. Not for use in diagnostic procedures.
Catalog Number A401
Catalog Number (CE) N/A
Size 250 µL
Price (USD) $250.00
Price (EURO) 220,00 €

Contact us

US Phone+1 (858) 552 1100
EU Phone+353 (91) 412 474
US Emailcontact-us@quidelortho.com
EU Emailcontact-emea@quidelortho.com

Specifications

Description

Purified human C3 protein

Size 250 µL
Storage

Store at -70°C or below. Avoid freeze/thaw (>10 % activity loss per thaw).

Form Frozen liquid. 10 mM sodium phosphate, 145 mM NaCl, pH 7.2. No preservative, 0.22 µm filtered.
Concentration 1.0 mg/mL (refer to CoA for lot specific concentration)
Applications See citations and technical data sheet for application info.
Purity >95% by SDS-PAGE
Source

Normal human serum

Activity >70% versus normal human serum standard
Molecular Weight 185,000 Da (2 chains)
Background

Purified human complement component C3. The third component of complement is a centrally important constituent of both the alternative and classical pathways. Upon secretion, pro-C3 undergoes a site specific proteolytic cleavage to yield circulating C3, a protein containing two disulfide bonded subunits (Alpha 115 kD and Beta 75 kD). The concentration of C3 in serum is approximately 1.25 +/– 0.52 mg/mL.

Citations

Title Year Applications Sample Species Sample Sample Details

Microglial-derived C1q integrates into neuronal ribonucleoprotein complexes and impacts protein homeostasis in the aging brain

2024

Liquid-liquid phase separation assay

Human

Purified Protein

Complement-Mediated Selective Tumor Cell Lysis Enabled by Bi-Functional RNA Aptamers.

2021

Binding Assay

Cell Culture

MDA-MB-231 Cells

Complement component 3 protects human bronchial epithelial cells from cigarette smoke-induced oxidative stress and prevents incessant apoptosis.

2022

Complement Activation Assay

Human

HBE Cells

Cigarette smoke-induced oxidative stress

Complement proteins bind to nanoparticle protein corona and undergo dynamic exchange in vivo.

2017

Dot Blot

Human

Serum

SPIO nanoworms

Alternative complement pathway deregulation is correlated with dengue severity

2009

ELISA

Human

Plasma

Dengue

Expression of complement components, receptors and regulators by human dendritic cells

2011

ELISA

Human

Dendritic Cell

Complement C3a and C5a modulate osteoclast formation and inflammatory response of osteoblasts in synergism with IL-1β

2011

ELISA

Human

Peripheral blood mononuclear cells

Properdin binding to complement activating surfaces depends on initial C3b deposition

2017

ELISA

Human

Serum

Extracellular vesicles from KSHV-infected endothelial cells activate the complement system

2017

ELISA

Human

HUVEC Cells

KSHV infection

Venom from Bothrops lanceolatus, a Snake Species Native to Martinique, Potently Activates the Complement System

2018

Silver Stain

Human

Purified protein

B. lanceolatus venom incubated

Inhibition of C3 Convertase Activity by Hepatitis C Virus as an Additional Lesion in the Regulation of Complement Components

2014

WB

Human

Serum

Complement protein C1q modulates neurite outgrowth in vitro and spinal cord axon regeneration in vivo

2015

ELISA

Mouse

Brain Tissue

Cerebellum myelin substrate

Complement Protein C3 Suppresses Axon Growth and Promotes Neuron Loss

2017

ELISA

Mouse

Neuron Cells

Presence of plasma complement regulatory proteins clusterin (Apo J) and vitronectin (S40) on circulating immune complexes (CIC)

2006

ELISA

N/A

Purified protein

Standard curve generation

The Inflammatory Feed-Forward Loop Triggered by the Complement Component C3 as a Potential Target in Endometriosis.

2021

WB

N/A

Control

Sensitivity and Specificity of plasma and urine complement split products as indicators of lupus disease activity

1996

WB

N/A

C3 Protein

A serine protease isolated from the bristles of the Amazonic caterpillar, Premolis semirufa, is a potent complement system activator.

2015

Silver Stain

Premolis semirufa (Caterpillar)

Bristle Extract

P-I snake venom metalloproteinase is able to activate the complement system by direct cleavage of central components of the cascade.

2013

Silver Stain

Snake

Bothrops pirajai venom