C3 Protein

Purified human C3 protein

Product Specifications

Citations	17
Description	Purified human C3 protein
Storage	Store at -70°C or below. Avoid freeze/thaw (>10 % activity loss per thaw).
Form	Frozen liquid. 10 mM sodium phosphate, 145 mM NaCl, pH 7.2. No preservative, 0.22 µm filtered.
Concentration	1.0 mg/mL (refer to CoA for lot specific concentration)
Applications	See citations and technical data sheet for application info.

Technical Documents

Ordering Information

For Research Use Only. Not for use in diagnostic procedures.

Catalog Number	A401
Catalog Number (CE)	N/A
Size	250 µL
Price (USD)	$250.00
Price (EURO)	220,00 €

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US Phone	+1 (858) 552 1100
EU Phone	+353 (91) 412 474
US Email	contact-us@quidelortho.com
EU Email	contact-emea@quidelortho.com

Specifications
Citations
Certificate of Analysis

Specifications

Description	Purified human C3 protein
Size	250 µL
Storage	Store at -70°C or below. Avoid freeze/thaw (>10 % activity loss per thaw).
Form	Frozen liquid. 10 mM sodium phosphate, 145 mM NaCl, pH 7.2. No preservative, 0.22 µm filtered.
Concentration	1.0 mg/mL (refer to CoA for lot specific concentration)
Applications	See citations and technical data sheet for application info.
Purity	>95% by SDS-PAGE
Source	Normal human serum
Activity	>70% versus normal human serum standard
Molecular Weight	185,000 Da (2 chains)
Background	Purified human complement component C3. The third component of complement is a centrally important constituent of both the alternative and classical pathways. Upon secretion, pro-C3 undergoes a site specific proteolytic cleavage to yield circulating C3, a protein containing two disulfide bonded subunits (Alpha 115 kD and Beta 75 kD). The concentration of C3 in serum is approximately 1.25 +/– 0.52 mg/mL.

Citations

Title	Year	Applications	Sample Species	Sample	Sample Details
Microglial-derived C1q integrates into neuronal ribonucleoprotein complexes and impacts protein homeostasis in the aging brain	2024	Liquid-liquid phase separation assay	Human	Purified Protein
Complement-Mediated Selective Tumor Cell Lysis Enabled by Bi-Functional RNA Aptamers.	2021	Binding Assay	Cell Culture	MDA-MB-231 Cells
Complement component 3 protects human bronchial epithelial cells from cigarette smoke-induced oxidative stress and prevents incessant apoptosis.	2022	Complement Activation Assay	Human	HBE Cells	Cigarette smoke-induced oxidative stress
Complement proteins bind to nanoparticle protein corona and undergo dynamic exchange in vivo.	2017	Dot Blot	Human	Serum	SPIO nanoworms
Alternative complement pathway deregulation is correlated with dengue severity	2009	ELISA	Human	Plasma	Dengue
Expression of complement components, receptors and regulators by human dendritic cells	2011	ELISA	Human	Dendritic Cell
Complement C3a and C5a modulate osteoclast formation and inflammatory response of osteoblasts in synergism with IL-1β	2011	ELISA	Human	Peripheral blood mononuclear cells
Properdin binding to complement activating surfaces depends on initial C3b deposition	2017	ELISA	Human	Serum
Extracellular vesicles from KSHV-infected endothelial cells activate the complement system	2017	ELISA	Human	HUVEC Cells	KSHV infection
Venom from Bothrops lanceolatus, a Snake Species Native to Martinique, Potently Activates the Complement System	2018	Silver Stain	Human	Purified protein	B. lanceolatus venom incubated
Inhibition of C3 Convertase Activity by Hepatitis C Virus as an Additional Lesion in the Regulation of Complement Components	2014	WB	Human	Serum
Complement protein C1q modulates neurite outgrowth in vitro and spinal cord axon regeneration in vivo	2015	ELISA	Mouse	Brain Tissue	Cerebellum myelin substrate
Complement Protein C3 Suppresses Axon Growth and Promotes Neuron Loss	2017	ELISA	Mouse	Neuron Cells
Presence of plasma complement regulatory proteins clusterin (Apo J) and vitronectin (S40) on circulating immune complexes (CIC)	2006	ELISA	N/A	Purified protein	Standard curve generation
The Inflammatory Feed-Forward Loop Triggered by the Complement Component C3 as a Potential Target in Endometriosis.	2021	WB	N/A	Control
Sensitivity and Specificity of plasma and urine complement split products as indicators of lupus disease activity	1996	WB	N/A	C3 Protein
A serine protease isolated from the bristles of the Amazonic caterpillar, Premolis semirufa, is a potent complement system activator.	2015	Silver Stain	Premolis semirufa (Caterpillar)	Bristle Extract
P-I snake venom metalloproteinase is able to activate the complement system by direct cleavage of central components of the cascade.	2013	Silver Stain	Snake	Bothrops pirajai venom

Certificate of Analysis

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