MicroVue™ CH50 Eq EIA

The MicroVue CH50 Eq EIA measures the total classical complement pathway activity in human serum and allows detection of a deficiency of one or more of the complement components C1 through C9.


Product Specifications

Citations 17
Specimen

Serum 14 μL

LLOQ N/A
ULOQ N/A
Assay Time 3.5 hours
Cross Reactivity

Cynomolgous monkey

Ordering Information

For In Vitro Diagnostic Use.
Catalog Number A018
Catalog Number (CE)  
Size 96 wells/test
Price (USD) $745.00
Price (EURO) 660,00 €

Contact us

US Phone+1 (858) 552 1100
EU Phone+353 (91) 412 474
US Emailcontact-us@quidelortho.com
EU Emailcontact-emea@quidelortho.com

Specifications

Description

The MicroVue CH50 Eq EIA measures the total classical complement pathway activity in human serum and allows detection of a deficiency of one or more of the complement components C1 through C9.

Size 96 wells/test
Form

96 well plate with 12 eight-well strips in a resealable foil pouch

Specimen Serum 14 μL
Limit of Detection (LOD) N/A
Lower Limit of Quantitation (LLOQ) N/A
Upper Limit of Quantitation (ULOQ) N/A
Intra Assay 3.2–4.5%
Inter Assay 5.4–8.7%
Standards 5
Controls 2
Sample Values

Normal 133±54 CH50 U Eq/mL

Assay Time 3.5 hours
Cross Reactivity

Cynomolgous monkey

Storage

Store the unopened kit at 2°C to 8°C. Refer to Product Insert for additional storage details.

Background

The binding of C1q component of C1 to immune complexes triggers the classical complement pathway. This activation results in a cascade of enzymatic and non-enzymatic reactions, culminating in the formation of terminal complement complexes (TCC). Under standard conditions the level of TCC that can be generated in a serum is a quantitative expression of the serum’s total classical complement activity. The traditional method for measuring the total classical complement activity in serum is the CH50 test. This test is a lytic assay, which uses antibody-sensitized sheep erythrocytes (EA) as the activator of the classical complement pathway and various dilutions of the test serum to determine the amount required to give 50% lysis. The percent hemolysis is determined spectrophotometrically. The CH50 test is an indirect measure of TCC, since the TCC themselves are directly responsible for the hemolysis that is measured. The MicroVue CH50 Eq EIA provides a direct measure of the total classical complement activity in serum by quantifying the amount of TCC generated under standard conditions. The MicroVue CH50 Eq EIA uses a monoclonal antibody to a unique neoantigen to capture the TCC analyte. Since both the CH50 Eq EIA and the CH50 test rely on the generation of TCC and correlate, the CH50 Eq EIA’s results are expressed in CH50 unit equivalents per milliliter.