MicroVue™ C5a EIA

The MicroVue C5a Enzyme Immunoassay is for the measurement of C5a in human serum, plasma, and other biological or experimental samples.


Product Specifications

Citations 57
Specimen

Serum 10 μL, EDTA or Citrated Plasma 20 μL

LLOQ 0.05 ng/mL
ULOQ N/A
Assay Time 2.5 hours
Cross Reactivity

None

Ordering Information

For Research Use Only in the United States. Not for use in diagnostic procedures.
Catalog Number A021
Catalog Number (CE) A025
Size 96 wells/test
Price (USD) $745.00
Price (EURO) 660,00 €

Contact us

US Phone+1 (858) 552 1100
EU Phone+353 (91) 412 474
US Emailcontact-us@quidelortho.com
EU Emailcontact-emea@quidelortho.com

Specifications

Description

The MicroVue C5a Enzyme Immunoassay is for the measurement of C5a in human serum, plasma, and other biological or experimental samples.

Size 96 wells/test
Form

96 well plate with 12 eight-well strips in a resealable foil pouch

Specimen Serum 10 μL, EDTA or Citrated Plasma 20 μL
Limit of Detection (LOD) 0.01 ng/mL
Lower Limit of Quantitation (LLOQ) 0.05 ng/mL
Upper Limit of Quantitation (ULOQ) N/A
Intra Assay 3.5–3.9%
Inter Assay 7.1–13%
Standards 5
Controls 2
Sample Values

Serum 13.4–179.2 ng/mL, EDTA Plasma 0.37–74.3 ng/mL

Assay Time 2.5 hours
Cross Reactivity

None

Storage

Store the unopened kit at 2°C to 8°C. Refer to Product Insert for additional storage details.

Background

The MicroVue C5a Enzyme Immunoassay is a 96 well, direct-capture immunoassay for the measurement of C5a in human serum, plasma, and other biological or experimental samples. Under normal conditions, activation of the classical, alternative, or lectin complement pathways results in the formation of a C5 convertase multi-molecular enzyme capable of cleaving C5 to C5a and C5b. C5b is a key constituent of the Terminal Complement Complex and has a variety of functions in this role. C5a is a low molecular weight (approximately 9kD) protein fragment of 74 amino acids. C5a is rapidly metabolized by the serum enzyme carboxypeptidase to a more stable, less active, 73 amino acid form, C5a des-Arg. For convenience, both forms will be referred to as “C5a” for purposes of this documentation. The MicroVue C5a assay, which provides a rapid, highly specific and quantitative procedure for measuring C5a levels, is designed for investigations into the role or status of terminal complement pathway activation in numerous research settings, and for monitoring the generation of C5a in vivo or in vitro. As the most potent of the complement anaphylatoxins, C5a has a host of biologic functions including mast cell degranulation, chemotaxis, leukosequestration, as well as cellular activation via binding to the C5a Receptor (C5aR or CD88). Research has associated elevated levels of fluid phase and adsorbed C5a with hemo-incompatibility of some biomaterials, particularly in extracorporeal circuits. Research has also associated levels of C5a with pathogenesis of a variety of disease states including myocardial infarction, stroke, as well as vascular leak syndrome and associated kidney injury. The role of C5a in the pathogenesis of malaria20 and other infectious diseases, as well as sepsis, is likewise well documented.