MicroVue™ C4a EIA

The MicroVue C4a Enzyme Immunoassay is for the measurement of C4a in human or primate serum, plasma, and other biological or experimental samples.


Product Specifications

Citations 19
Specimen

Serum/EDTA Plasma 10 μL

LLOQ 5.0 ng/mL
ULOQ 61 ng/mL
Assay Time 2.5 hours
Cross Reactivity

African Green Monkey, Cynomolgous monkey, Rhesus monkey

Ordering Information

For Research Use Only in the United States. Not for use in diagnostic procedures.
Catalog Number A035
Catalog Number (CE) A036
Size 96 wells/test
Price (USD) $745.00
Price (EURO) 660,00 €

Contact us

US Phone+1 (858) 552 1100
EU Phone+353 (91) 412 474
US Emailcontact-us@quidelortho.com
EU Emailcontact-emea@quidelortho.com

Specifications

Description

The MicroVue C4a Enzyme Immunoassay is for the measurement of C4a in human or primate serum, plasma, and other biological or experimental samples.

Size 96 wells/test
Form

96 well plate with 12 eight-well strips in a resealable foil pouch

Specimen Serum/EDTA Plasma 10 μL
Limit of Detection (LOD) 0.29 ng/mL
Lower Limit of Quantitation (LLOQ) 5.0 ng/mL
Upper Limit of Quantitation (ULOQ) 61 ng/mL
Intra Assay 3.7–4.3%
Inter Assay 4.0–4.4%
Standards 5
Controls 2
Sample Values

Serum 20.9–4437.2 ng/mL, EDTA Plasma 383.5–8168.2 ng/mL

Assay Time 2.5 hours
Cross Reactivity

African Green Monkey, Cynomolgous monkey, Rhesus monkey

Storage

Store the unopened kit at 2°C to 8°C. Refer to Product Insert for additional storage details.

Background

Under normal conditions, activation of the classical or lectin complement pathways results in the cleavage of the complement protein, C4 into C4a and C4b by the protease, C1s. C4a is rapidly cleaved to its more stable, less active form C4a-des Arg form by endogenous serum carboxypeptidase N enzyme. Thus, quantitation of C4a-des Arg should provide a reliable measurement of classical or lectin complement pathway activation that has occurred in test samples. The MicroVue C4a assay, a rapid, highly specific and quantitative procedure for measuring C4a levels, is designed for investigations into the role or status of complement pathway activation in numerous research settings, and for monitoring the generation of C4a in vivo or in vitro. C4a is the weakest of the anaphylatoxins, compared with C3a and C5a, however, it does play a role in bringing about vascular permeability changes, the induction of smooth muscle contraction and the release of histamine from mast cells and basophils. C4a is believed to play a role in several autoimmune diseases including rheumatoid arthritis, SLE and acute glomerulonephritis. It has recently been implicated as a marker for both acute and chronic Lyme disease.