MicroVue™ Complement Multiplex

ELISA based chemiluminescent multiplex for complement system analytes.


Product Specifications

 

Citations16
Specimen

Plasma, Serum

LLOQVaries
ULOQVaries
Assay Time3.5 Hours
Cross Reactivity

Rhesus Monkey, Cynomolgus Monkey 

Ordering Information

Intended Use: For research use only in the United States. Not for use in diagnostic procedures

Catalog Number

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Catalog Number (xUS)

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Size96 wells/test
Price (USD)

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Price (EURO)

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Contact us

US Phone+1 (858) 552 1100
EU Phone+353 (91) 412 474
US Emailcontact-us@quidelortho.com
EU Emailcontact-emea@quidelortho.com

Product description

 
An ELISA based assay designed to generate maximum data

The MicroVue Complement Multiplex is an ELISA based platform that allows for the measurement of up to 10 different complement system proteins. QuidelOrtho offers the following assay options (see the Ordering tab for more information):

How it works
ELISA within ELISA
  • Capture antibody is applied to 350-500 μm spots to bind target proteins.
  • Robotic liquid handlers deposit 20-50 nL of print diluent.
  • Static 96 well plate specially created to prevent inter-well signal loss.
Analyte Detection

Signal from individual spots is generated via standard biotin and streptavidin-HRP complexing. Chemiluminescent signal detection and intensity is then used to determine presence and quantity of the specific analytes.

For more information, view the MicroVue Complement Mulitplex brochure for full product specifications, performance review, technology overview, and more.

View our MicroVue Complement Multiplex advantages flyer for how the platform increase laboratory productivity.

Available analytes
Panel 1Panel 2
BaC1q
BbC2 intact
C2 intactC3 intact
C3aC3 intact
C3dC5 intact
C4aFactor D
C4dFactor P
C5a-
sC5b-9-
Factor D-
Factor H-
Factor I-
Why 2 Panels?

Complement proteins are found at different concentrations in the blood. Panel 1 analytes require a lower dilution factor (1:100) than those in Panel 2 (1:1000) for the multiplex to perform properly.

  • C2 Intact and Factor D can be utilized within both panels.
  • All other analytes must be utilized within their respective panels.
Compatability

A limited selection of analytes are not compatible for Panel 1

  • C3a is not compatible with C3d.
  • C4a is not compatible with C4d.

All Panel 2 analytes are compatible