MicroVue™ Osteocalcin EIA
The MicroVue Osteocalcin immunoassay measures intact (de novo) osteocalcin in serum.
Product Specifications
| Citations | 7 |
|---|---|
| Specimen |
Serum 25 μL |
| LLOQ | N/A |
| ULOQ | N/A |
| Assay Time | 3.6 hours |
| Cross Reactivity |
Cow, Cynomolgous monkey, Guinea Pig, Horse, Goat, Pig, Rabbit, Sheep |
Ordering Information
| Catalog Number | 8001 |
|---|---|
| Catalog Number (CE) | 8002 |
| Size | 96 wells/test |
| Price (USD) | $660.00 |
| Price (EURO) | 590,00 € |
Contact us
| US Phone | +1 (858) 552 1100 |
|---|---|
| EU Phone | +353 (91) 412 474 |
| US Email | contact-us@quidelortho.com |
| EU Email | contact-emea@quidelortho.com |
- Specifications
- Citations
- Certificate of Analysis
Specifications
| Description |
The MicroVue Osteocalcin immunoassay measures intact (de novo) osteocalcin in serum. |
|---|---|
| Size | 96 wells/test |
| Form |
96 well plate with 12 eight-well strips in a resealable foil pouch |
| Specimen | Serum 25 μL |
| Limit of Detection (LOD) | 0.45 ng/mL |
| Lower Limit of Quantitation (LLOQ) | N/A |
| Upper Limit of Quantitation (ULOQ) | N/A |
| Intra Assay | 4.8–10.0% |
| Inter Assay | 4.8–9.8% |
| Standards | 6 |
| Controls | 2 |
| Sample Values |
Adult: 3.7–10.0 ng/mL |
| Assay Time | 3.6 hours |
| Cross Reactivity |
Cow, Cynomolgous monkey, Guinea Pig, Horse, Goat, Pig, Rabbit, Sheep |
| Storage |
Store unopened kit between 2°C to 8°C. Refer to manual for storing individual components |
| Background |
Osteocalcin (OC) or BGP (bone gla protein) is found exclusively in bone tissue. It is a 5800 molecular weight extrahepatic vitamin K dependent protein produced by osteoblasts. It contains three gamma-carboxyglutamic acid residues which are thought to be involved in calcium ion and hydroxyapatite binding. It accounts for 10%-20% of the noncollagenous protein in bone. While the in vivo function of osteocalcin is unknown, its affinity for bone mineral constituents implies a role in bone formation. A Highly Specific Assay for Intact OC 1) Does not detect carboxy- or aminoterminal peptides, 2) Requires tertiary structure for antibody recognition, and 3) Detects intact OC, not OC fragments released during osteoclastic resorption. |
Citations
| Title | Year | Applications | Sample Species | Sample | Sample Details |
|---|---|---|---|---|---|
| 2011 | ELISA |
Human |
Mesenchymal Cells |
||
| 2012 | ELISA |
Human |
Serum |
||
|
Higher daily physical activity is associated with higher osteocalcin levels in adolescents. |
2015 | ELISA |
Human |
Serum |
Obesity, children |
| 2016 | ELISA |
Human |
Serum |
||
| 2019 | ELISA |
Human |
Urine |
||
| 2019 | ELISA |
Human |
Serum |
||
| 2019 | ELISA |
Mouse |
Plasma |
Certificate of Analysis
