C5a Protein

Purified human C5a protein


Product Specifications

Citations 0
Description

Purified human C5a protein

Storage Store at -70°C or below. Avoid freeze/thaw.
Form

Frozen liquid. 20 mM HEPES, 100 mM NaCl, pH 7.2. (No carrier proteins added). No preservative.

Concentration 0.5 mg/mL (refer to CoA for lot specific concentration)
Applications

See citations and technical data sheet for application info.

Ordering Information

For Research Use Only. Not for use in diagnostic procedures.
Catalog Number A420
Catalog Number (CE) N/A
Size 60 µL
Price (USD) $545.00
Price (EURO) 430,00 €

Contact us

US Phone+1 (858) 552 1100
EU Phone+353 (91) 412 474
US Emailcontact-us@quidelortho.com
EU Emailcontact-emea@quidelortho.com

Specifications

Description

Purified human C5a protein

Size 60 µL
Storage

Store at -70°C or below. Avoid freeze/thaw.

Form Frozen liquid. 20 mM HEPES, 100 mM NaCl, pH 7.2. (No carrier proteins added). No preservative.
Concentration 0.5 mg/mL (refer to CoA for lot specific concentration)
Applications See citations and technical data sheet for application info.
Purity >97% by SDS-PAGE
Source

Normal human serum

Activity >70% by myeloperoxidase release assay
Molecular Weight 10,400 Da (single chain)
Background

Natural human C5a is prepared from human C5 protein by cleavage of the peptide bond between C5a and C5b by the human C5 convertase. C5a is a naturally glycosylated polypeptide containing 74 amino acids with a molecular weight of approx. 10,400 daltons. It contains 25% carbohydrate attached to a single Asn residue at position 64. This carbohydrate is of variable structure leading to a broad distribution of MW upon analysis by mass spectroscopy. C5a is the most potent anaplylatoxin (compared to C3a and C4a). Its biological properties include being strongly chemotactic for neutrophils (PMN), causing smooth muscle contraction, increasing vascular permeability, causing histamine and TNF-alpha release, and causing lysosomal degranulation of immune cells. C5a acts through the C5a Receptor (C5aR, CD88, a G-protein coupled receptor) on PMN, monocytes, alveolar macrophages, and mast cells.

Citations

Title Year Applications Sample Species Sample Sample Details