C4b Protein
Purified human C4b protein
Product Specifications
| Citations | 0 |
|---|---|
| Description |
Purified human C4b protein |
| Storage | Store at -70°C or below. Avoid freeze/thaw. |
| Form |
Frozen liquid. 10 mM sodium phosphate, 145 mM NaCl, pH 7.3. No preservative, 0.22 µm filtered. |
| Concentration | 1.0 mg/mL (refer to CoA for lot specific concentration) |
| Applications |
See citations and technical data sheet for application info. |
Ordering Information
| Catalog Number | A428 |
|---|---|
| Catalog Number (CE) | N/A |
| Size | 250 µL |
| Price (USD) | $260.00 |
| Price (EURO) | 210,00 € |
Contact us
| US Phone | +1 (858) 552 1100 |
|---|---|
| EU Phone | +353 (91) 412 474 |
| US Email | contact-us@quidelortho.com |
| EU Email | contact-emea@quidelortho.com |
- Specifications
- Citations
- Certificate of Analysis
Specifications
| Description |
Purified human C4b protein |
|---|---|
| Size | 250 µL |
| Storage |
Store at -70°C or below. Avoid freeze/thaw. |
| Form | Frozen liquid. 10 mM sodium phosphate, 145 mM NaCl, pH 7.3. No preservative, 0.22 µm filtered. |
| Concentration | 1.0 mg/mL (refer to CoA for lot specific concentration) |
| Applications | See citations and technical data sheet for application info. |
| Purity | >90% by SDS-PAGE |
| Source |
Normal human serum |
| Activity | No activity |
| Molecular Weight | 176,000 Da (2 chains) |
| Background |
C4b is derived from native C4 upon cleavage and release of C4a. It is prepared by cleavage of purified C4 with the classical pathway activated protease C1s enzyme (#A104). Native human C4b is a glycosylated (~7.3%) polypeptide containing three disulfide-linked chains. C4b is central to the function of two of the three pathways of complement. Both the classical and lectin pathways of complement activation generate surface-bound active proteases that cleave C4 (C1s in the classical pathway and MASP1/2 in the lectin pathway). These enzymes cleave a peptide bond in C4 releasing the anaphylatoxin C4a and activating C4b. For a fraction of a second after proteolytic activation C4b can covalently attach to the surface of the target. |
Citations
| Title | Year | Applications | Sample Species | Sample | Sample Details |
|---|
Certificate of Analysis
