MicroVue™ Factor I EIA

The MicroVue Factor I EIA is an enzyme immunoassay for the quantitative measurement of complment Factor I.


Product Specifications

Citations 0
Specimen

Serum/EDTA Plasma 10 μL

LLOQ 1.6 ng/mL
ULOQ 82.1 ng/mL
Assay Time 2.5 hours
Cross Reactivity

None

Ordering Information

For Research Use Only in the United States. Not for use in diagnostic procedures.
Catalog Number A041
Catalog Number (CE)  
Size 96 wells/test
Price (USD) $725.00
Price (EURO) 650,00 €

Contact us

US Phone+1 (858) 552 1100
EU Phone+353 (91) 412 474
US Emailcontact-us@quidelortho.com
EU Emailcontact-emea@quidelortho.com

Specifications

Description

The MicroVue Factor I EIA is an enzyme immunoassay for the quantitative measurement of complment Factor I.

Size 96 wells/test
Form

96 well plate with 12 eight-well strips in a resealable foil pouch

Specimen Serum/EDTA Plasma 10 μL
Limit of Detection (LOD) 0.5 ng/mL
Lower Limit of Quantitation (LLOQ) 1.6 ng/mL
Upper Limit of Quantitation (ULOQ) 82.1 ng/mL
Intra Assay 3%
Inter Assay 5%
Standards 5
Controls 2
Sample Values

None

Assay Time 2.5 hours
Cross Reactivity

None

Storage

Store the unopened kit at 2°C to 8°C. Refer to Product Insert for additional storage details.

Background

Factor I (FI) is a negative regulatory protein of the complement system, and inhibits all pathways by cleaving activation components C4b and C3b. FI is a soluble protein, and circulates freely in the blood, though in an inactive state. Once FI is in the presence of a variety of co-factors it activates and inhibits complement activation. These co-factors include Factor H (only for C3b cleavage), MCP (CD46), CD35, and C4BP. The MicroVue Factor I EIA measures the concentration of Factor I in serum or plasma. It uses a monoclonal antibody to Factor I, which is subsequently detected with an HRP-conjugated antibody that bind to another epitope of Factor I. This test, which provides a rapid, highly specific and quantitative procedure for measuring Factor I levels, is designed for investigations studying the role or status of complement pathway regulation in numerous research settings.

Citations

Title Year Applications Sample Species Sample Sample Details