MicroVue™ SC5b-9 Plus EIA

The MicroVue SC5b-9 Plus Enzyme Immunoassay measures the amount of the SC5b-9 complex in specimens.


Product Specifications

Citations 97
Specimen

Serum 10 μL, EDTA or Citrated Plasma 50 μL

LLOQ 8.8 ng/mL
ULOQ N/A
Assay Time 2 hours
Cross Reactivity

African Green Monkey, Baboon, Cynomolgous monkey, Pigtail Monkey, Rabbit, Rhesus monkey

Ordering Information

For Research Use Only in the United States. Not for use in diagnostic procedures.
Catalog Number A020
Catalog Number (CE) A029
Size 96 wells/test
Price (USD) $745.00
Price (EURO) 670,00 €

Contact us

US Phone+1 (858) 552 1100
EU Phone+353 (91) 412 474
US Emailcontact-us@quidelortho.com
EU Emailcontact-emea@quidelortho.com

Specifications

Description

The MicroVue SC5b-9 Plus Enzyme Immunoassay measures the amount of the SC5b-9 complex in specimens.

Size 96 wells/test
Form

96 well plate with 12 eight-well strips in a resealable foil pouch

Specimen Serum 10 μL, EDTA or Citrated Plasma 50 μL
Limit of Detection (LOD) 3.7 ng/mL
Lower Limit of Quantitation (LLOQ) 8.8 ng/mL
Upper Limit of Quantitation (ULOQ) N/A
Intra Assay 1.6–6.8%
Inter Assay 5.0–13.1%
Standards 5
Controls 2
Sample Values

None

Assay Time 2 hours
Cross Reactivity

African Green Monkey, Baboon, Cynomolgous monkey, Pigtail Monkey, Rabbit, Rhesus monkey

Storage

Store the unopened kit at 2°C to 8°C. Refer to Product Insert for additional storage details.

Background

The Terminal Complement Complex (TCC, SC5b-9) is generated by the assembly of C5 through C9 as a consequence of activation of the complement system by either the classical, lectin or alternative pathway. The membrane attack complex (MAC), a form of TCC, is a stable complex that mediates the irreversible target cell membrane damage associated with complement activation. Complexes formed in the absence of a target membrane bind to naturally occurring regulatory serum proteins, e.g. the S protein, at the C5b-7 stage of assembly forming soluble, non-lytic TCC. For purposes of this document, we refer to all forms of stable Terminal Complement Complex interchangeably as TCC and SC5b-9, recognizing that other complement regulatory proteins, like Clusterin, also form these stable complexes and are detectable in the SC5b-9 Plus assay. The MicroVue SC5b-9 Plus Enzyme Immunoassay measures the concentration of TCC thereby giving an indication of the status of the terminal complement pathway in the specimen. It uses a monoclonal antibody to the C9 ring of TCC to capture the complex. The trapped TCC is subsequently detected with HRP-conjugated antibodies that bind to antigens of the SC5b-9 complex. This test, which provides a rapid, highly specific and quantitative procedure for measuring TCC levels, is designed for investigations studying the role or status of terminal complement pathway activation in numerous research settings, and for monitoring the generation of SC5b-9 complexes in vivo or in vitro.