MicroVue™ Factor I EIA

The MicroVue Factor I EIA is an enzyme immunoassay for the quantitative measurement of complment Factor I.


Product Specifications

Citations0
Specimen

Serum/EDTA Plasma 10 μL

LLOQ1.6 ng/mL
ULOQ82.1 ng/mL
Assay Time2.5 hours
Cross Reactivity

None

Ordering Information

For Research Use Only in the United States. Not for use in diagnostic procedures.
Catalog NumberA041
Catalog Number (CE) 
Size96 wells/test
Price (USD)$725.00
Price (EURO)650,00 €

Contact us

US Phone+1 (858) 552 1100
EU Phone+353 (91) 412 474
US Emailcontact-us@quidelortho.com
EU Emailcontact-emea@quidelortho.com

Specifications

Description

The MicroVue Factor I EIA is an enzyme immunoassay for the quantitative measurement of complment Factor I.

Size96 wells/test
Form

96 well plate with 12 eight-well strips in a resealable foil pouch

SpecimenSerum/EDTA Plasma 10 μL
Limit of Detection (LOD)0.5 ng/mL
Lower Limit of Quantitation (LLOQ)1.6 ng/mL
Upper Limit of Quantitation (ULOQ)82.1 ng/mL
Intra Assay3%
Inter Assay5%
Standards5
Controls2
Sample Values

None

Assay Time2.5 hours
Cross Reactivity

None

Storage

Store the unopened kit at 2°C to 8°C. Refer to Product Insert for additional storage details.

Background

Factor I (FI) is a negative regulatory protein of the complement system, and inhibits all pathways by cleaving activation components C4b and C3b. FI is a soluble protein, and circulates freely in the blood, though in an inactive state. Once FI is in the presence of a variety of co-factors it activates and inhibits complement activation. These co-factors include Factor H (only for C3b cleavage), MCP (CD46), CD35, and C4BP. The MicroVue Factor I EIA measures the concentration of Factor I in serum or plasma. It uses a monoclonal antibody to Factor I, which is subsequently detected with an HRP-conjugated antibody that bind to another epitope of Factor I. This test, which provides a rapid, highly specific and quantitative procedure for measuring Factor I levels, is designed for investigations studying the role or status of complement pathway regulation in numerous research settings.

Citations

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