C5 Antibody (Monoclonal) , Biotinylated

A biotin conjugated murine monoclonal antibody to an epitope on C5.

250 µL

Purified human protein.

IgG1k

≥ 95% by SDS PAGE

This monoclonal antibody was raised against purified human C5. It is specific for an antigen expressed on the C5 but not on MAC Complex. This antibody inhibits the lysis of EA and will not bind to MAC bound to a membrane.

Short term (30 days) 4˚C. Long term at or below –20˚C.

Complement protein, C5 is unique to the Terminal Complement Pathway. The C5 protein is composed of two subunits (Alpha – 115,000 kD and Beta – 75,000 kD) and is present in serum at a concentration of approximately 70 mg/L. Under normal conditions, activation of either the Classical, Lectin or Alternative Complement Pathways generates the C5 convertase which is capable of cleaving C5 into two fragments – C5a (a potent anaphylatoxin) and C5b. Together with other proteins of the Terminal Pathway, C5b forms the Membrane Attack Complex (MAC) or Terminal Complement Complex (TCC) at the surface of a target cell; resulting in cell lysis.