C3 Protein
Purified human C3 protein
Product Specifications
| Citations | 17 |
|---|---|
| Description | Purified human C3 protein |
| Storage | Store at -70°C or below. Avoid freeze/thaw (>10 % activity loss per thaw). |
| Form | Frozen liquid. 10 mM sodium phosphate, 145 mM NaCl, pH 7.2. No preservative, 0.22 µm filtered. |
| Concentration | 1.0 mg/mL (refer to CoA for lot specific concentration) |
| Applications | See citations and technical data sheet for application info. |
Ordering Information
| Catalog Number | A401 |
|---|---|
| Catalog Number (CE) | N/A |
| Size | 250 µL |
| Price (USD) | $250.00 |
| Price (EURO) | 220,00 € |
Contact us
| US Phone | +1 (858) 552 1100 |
|---|---|
| EU Phone | +353 (91) 412 474 |
| US Email | contact-us@quidelortho.com |
| EU Email | contact-emea@quidelortho.com |
- Specifications
- Citations
- Certificate of Analysis
Specifications
| Description | Purified human C3 protein |
|---|---|
| Size | 250 µL |
| Storage | Store at -70°C or below. Avoid freeze/thaw (>10 % activity loss per thaw). |
| Form | Frozen liquid. 10 mM sodium phosphate, 145 mM NaCl, pH 7.2. No preservative, 0.22 µm filtered. |
| Concentration | 1.0 mg/mL (refer to CoA for lot specific concentration) |
| Applications | See citations and technical data sheet for application info. |
| Purity | >95% by SDS-PAGE |
| Source | Normal human serum |
| Activity | >70% versus normal human serum standard |
| Molecular Weight | 185,000 Da (2 chains) |
| Background | Purified human complement component C3. The third component of complement is a centrally important constituent of both the alternative and classical pathways. Upon secretion, pro-C3 undergoes a site specific proteolytic cleavage to yield circulating C3, a protein containing two disulfide bonded subunits (Alpha 115 kD and Beta 75 kD). The concentration of C3 in serum is approximately 1.25 +/– 0.52 mg/mL. |
Citations
| Title | Year | Applications | Sample Species | Sample | Sample Details |
|---|---|---|---|---|---|
| 2022 | Complement Activation Assay | Human | HBE Cells | Cigarette smoke-induced oxidative stress | |
Complement-Mediated Selective Tumor Cell Lysis Enabled by Bi-Functional RNA Aptamers. | 2021 | Binding Assay | Cell Culture | MDA-MB-231 Cells | N/A |
| 2021 | WB | N/A | Control | N/A | |
| 2018 | Silver Stain | Human | Purified protein | B. lanceolatus venom incubated | |
Complement proteins bind to nanoparticle protein corona and undergo dynamic exchange in vivo. | 2017 | Dot Blot | Human | Serum | SPIO nanoworms |
Extracellular vesicles from KSHV-infected endothelial cells activate the complement system | 2017 | ELISA | Human | HUVEC Cells | KSHV infection |
Properdin binding to complement activating surfaces depends on initial C3b deposition | 2017 | ELISA | Human | Serum | N/A |
Complement Protein C3 Suppresses Axon Growth and Promotes Neuron Loss | 2017 | ELISA | Mouse | Neuron Cells | N/A |
| 2015 | ELISA | Mouse | Brain Tissue | Cerebellum myelin substrate | |
| 2015 | Silver Stain | Premolis semirufa (Caterpillar) | Bristle Extract | N/A | |
| 2014 | WB | Human | Serum | ||
| 2013 | Sliver Stain | Snake | Bothrops pirajai venom | N/A | |
Expression of complement components, receptors and regulators by human dendritic cells | 2011 | ELISA | Human | Dendritic Cell | N/A |
| 2011 | ELISA | Human | Peripheral blood mononuclear cells | N/A | |
Alternative complement pathway deregulation is correlated with dengue severity | 2009 | ELISA | Human | Plasma | Dengue |
| 2006 | ELISA | N/A | Purified protein | Standard curve generation | |
| 1996 | WB | N/A | C3 Protein | N/A |
Certificate of Analysis
