C5a Protein
Purified human C5a protein
Product Specifications
| Citations | 0 |
|---|---|
| Description |
Purified human C5a protein |
| Storage | Store at -70°C or below. Avoid freeze/thaw. |
| Form |
Frozen liquid. 20 mM HEPES, 100 mM NaCl, pH 7.2. (No carrier proteins added). No preservative. |
| Concentration | 0.5 mg/mL (refer to CoA for lot specific concentration) |
| Applications |
See citations and technical data sheet for application info. |
Ordering Information
| Catalog Number | A420 |
|---|---|
| Catalog Number (CE) | N/A |
| Size | 60 µL |
| Price (USD) | $545.00 |
| Price (EURO) | 430,00 € |
Contact us
| US Phone | +1 (858) 552 1100 |
|---|---|
| EU Phone | +353 (91) 412 474 |
| US Email | contact-us@quidelortho.com |
| EU Email | contact-emea@quidelortho.com |
- Specifications
- Citations
- Certificate of Analysis
Specifications
| Description |
Purified human C5a protein |
|---|---|
| Size | 60 µL |
| Storage |
Store at -70°C or below. Avoid freeze/thaw. |
| Form | Frozen liquid. 20 mM HEPES, 100 mM NaCl, pH 7.2. (No carrier proteins added). No preservative. |
| Concentration | 0.5 mg/mL (refer to CoA for lot specific concentration) |
| Applications | See citations and technical data sheet for application info. |
| Purity | >97% by SDS-PAGE |
| Source |
Normal human serum |
| Activity | >70% by myeloperoxidase release assay |
| Molecular Weight | 10,400 Da (single chain) |
| Background |
Natural human C5a is prepared from human C5 protein by cleavage of the peptide bond between C5a and C5b by the human C5 convertase. C5a is a naturally glycosylated polypeptide containing 74 amino acids with a molecular weight of approx. 10,400 daltons. It contains 25% carbohydrate attached to a single Asn residue at position 64. This carbohydrate is of variable structure leading to a broad distribution of MW upon analysis by mass spectroscopy. C5a is the most potent anaplylatoxin (compared to C3a and C4a). Its biological properties include being strongly chemotactic for neutrophils (PMN), causing smooth muscle contraction, increasing vascular permeability, causing histamine and TNF-alpha release, and causing lysosomal degranulation of immune cells. C5a acts through the C5a Receptor (C5aR, CD88, a G-protein coupled receptor) on PMN, monocytes, alveolar macrophages, and mast cells. |
Citations
| Title | Year | Applications | Sample Species | Sample | Sample Details |
|---|
Certificate of Analysis
